http://dr.lib.sjp.ac.lk/handle/123456789/762 2026-04-23T10:23:45Z http://dr.lib.sjp.ac.lk/handle/123456789/1043 Studies on Effect of Flabelliferins and Dietary Fibre on the Hypocholesterolaemic Effect of Palmyrah Fruit Pulp Pathberiya, L.G.; Jansz, E.R. The fruit pulp of all 4 main types of Palmyrah (Borassus flabellifer L.,J has been previously shown to have a significant hypocholesterolaemic effect in ICR mice. Beta-sitosterol. the parent sterol of flabelliferins, is known to reduce cholesterol uptake but is not present in fresh PFP and these studies indicate that it cannot be formed in the small intestine. In-vivo and In-vitro studies show that colonic bacteria can hydrolyse flabelliferins but this is not a significant source of absorbable p- Sitiosterol. Hydrolysis offlabelliferins with nariginase to shorten the carbohydrate moiety results in no significant differences in serum cholesterol on feeding to mice compared to the non-hydrolysed PFP (P=0.78) showing that small carbohydrate chain flabelliferins cannot mimic p-sitoserol action in lowering cholesterol absorption. The study of dietary fibre of 4 types of PFP show that content is high (12.3-24.3% dryweign) but variable as is the ratio of soluble and insoluble dietary fibre. The bile salt content offaeces of mice fed on 10% PFP feed measured using enzymatic colorimetric assay results in significantly higher bile salts (67%) compared to the control in faeces. (p=0.0009). 2005-01-01T00:00:00Z http://dr.lib.sjp.ac.lk/handle/123456789/1042 Studies on Dietary Fibre Content of Four Uncommon Palmyrah Fruit Types Seneviratne, R.M.S.K.; Johansson, Magnus; Pathberiya, L.Gayathri; Jansz, E.R. The fruit pulp of common types of palmyrah (Borassus flabellifer) contains high quantities of dietary fibre and pectin content which had been reported to be 5-7%. Four uncommon fruit types were collected from Mannar, Sri Lanka, The fruit pulp contained 12,8 to 16.8% insoluble dietary fibre and 9.7 to 10.9% dry weight soluble dietary fibre. Pectin contents were high (8.1 to 10.6% dry weight) and pectin was by far the largest contributor soluble dietary fibre. Sepharose-gel chromatography showed that the soluble dietary fibre was polydisperse with some factions in type A and type B showing eluent volume corresponding to molecular weight in excess of2 million Daltons. The study show that: (i) if these uncommon type are propagated this could yield a commercially valuable by-product in pectin. (ii) high fibre content of fruit pulp can result in medically beneficial effects when edible palmyrah fruit preparations is consumed. 2005-01-01T00:00:00Z http://dr.lib.sjp.ac.lk/handle/123456789/1041 Status of Rainbow Trout ( Oncorhynchus mykiss-Waldaum 1792) Population after the Cessation of Stocking in Waterways of Horton Plains, Sri Lanka Jinadasa, J.; Kothalawala, A.B.; Herath, H.M.B.K. The rainbow trout (Oncorhynchus mykiss) was originally introduced to Sri Lanka as a sport fish in 1882, then it was called Salmo gairdneri and reported to be surviving in few numbers only in the Horton Plaints, although, the regular stocking ceased in 1989. This study is concentrated into determine the status of the present population. The study consisted of regular sampling period during April 1998 to March 1999 and occasional investigations in 2000 and 2004. The Rainbow trout, size ranging from 1.0 to 39.0 em, mature population isjust about 1000 in the Horton plaints and they exhibited size dependent distribution over different stream habitats. For breeding they migrate to first order streams where fry and fingering can be found as well. Spawning period is from late December to early March in a year when the temperature was lowest. High egg mortality between 40% and 78% was observed due to poor water quality. Average length of fish caught in each station indicated downstream increase. Surface water temperature and pH had positive relationship while dissolved oxygen content and total dissolved solid showed negative relationship with downstream direction. The slit load in water is high in higher order streams. The populations examined were devoid in headwater streams other than those ofBelihul Oya. 2005-01-01T00:00:00Z http://dr.lib.sjp.ac.lk/handle/123456789/1040 Purification and Partial Characterisation of Glycosylated Bovine Alpha Lactalbumin Chandrika, U.G. Bovine alpha lactalbumin exists in four different forms. These are F, M, S, and S,' so named because of relative position on non-denaturing polysaccharide gels. F, S, and S, are minor components that make up 15% ofthe total alpha lactalbumin and S, and S' have shown to be glycoforms of protein. The lectin affinity column chromatography (Concanvalin A ) was used to seprate glycosylated protein from non glycosyalated contaminants from acid whey. Size exclusion chromatography was then used to separate the glycosylated alpha lactalbumin from the immunoglobulins and other contamnants. In order to analyse the number of oligosaccharide chains bound to protein, they were enzymatically cleaved from the protein using peptide-N4-(N-acetyl-(3-glucosamnyl) asparagines aminase (PNGase F), then separated using high pH anion exchange chromatography with pulsed amperometic detection (HPEA/PAD) and tluorophore assisted carbohydrate electrophoresis (FACE). Electrospray mass spectroscopy was used to confirm the results of these techniques. The structure of the main sialic acid containing glycan and neural glycan wete postulated, which represent S, and S, respectively. It is interesting to note that these molecules differs only one sialic acid residue. 2005-01-01T00:00:00Z