In vitro plant regeneration of Stevia rebaudiana through indirect organogenesis

dc.contributor.author	Gunasena, M.D.K.M
dc.contributor.author	Senarath, W.T.P.S.K
dc.date.accessioned	2022-02-11T03:58:02Z
dc.date.available	2022-02-11T03:58:02Z
dc.date.issued	2019
dc.identifier.citation	Gunasena, M.D.K.M., Senarath, W.T.P.S.K.(2019)."In vitro plant regeneration of Stevia rebaudiana through indirect organogenesis", International Journal of Botany Studies, Volume 4; Issue 4; July 2019; Page No. 199-203	en_US
dc.identifier.uri	http://dr.lib.sjp.ac.lk/handle/123456789/10241
dc.description.abstract	Stevia rebaudiana plants produce non caloric sweeteners that can be used as an alternative to sugar. In the present study reliable protocol was developed to obtain healthy plants of S. rebaudiana through callus cultures. Leaf discs and nodal explants were cultured on MS medium supplemented with different concentrations and combinations of PGRs. MS medium supplemented with 2.0 mg/L BAP and 1.0 mg/L NAA was found to be the best medium for callus induction. Leaf disc was found to be the best explant type for callus initiation. Leaf derived calli were transferred into shoot induction media and MS medium supplemented with 0.5 mg/L NAA and 2.0 mg/L BAP was the best medium. Shoots with more than 5.0 cm were transferred into root induction media and half strength MS medium supplemented with 0.5 mg/L IBA was the best medium for root induction.	en_US
dc.language.iso	en	en_US
dc.publisher	International Journal of Botany Studies	en_US
dc.subject	Stevia rebaudiana, callus induction, shoot induction, root induction, explants, organogenesis	en_US
dc.title	In vitro plant regeneration of Stevia rebaudiana through indirect organogenesis	en_US
dc.type	Article	en_US