Abstract:
Amplification of random regions of genomic DNA using 10-base primers in the
randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)
was used to identify the genetic polymorphisms of Ae. aegypti mosquito
populations based on genetic variation. Genomic DNA was extracted from
individual mosquitoes from 10 populations of Ae. aegypti and amplified in PCR
reaction using single primers of arbitrary nucleotide sequence. RAPD fragments
were produced using 8 different primers. The genetic relatedness of the populations
were estimated using Nei and Li pair-wise genetic distances similarity index
analysis.