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Diagnosing Cutaneous leishmaniasis using Fluorescence in Situ Hybridization: the Sri Lankan Perspective

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dc.contributor.author Kaluarachchi, T.D.J.
dc.contributor.author Weerasekera, M.M
dc.contributor.author McBain, A.J
dc.contributor.author Yasawardene, S.
dc.date.accessioned 2022-08-08T07:59:58Z
dc.date.available 2022-08-08T07:59:58Z
dc.date.issued 2019
dc.identifier.citation Kaluarachchi, T.D.J., et al. (2019). Diagnosing Cutaneous leishmaniasis using Fluorescence in Situ Hybridization: the Sri Lankan Perspective. PATHOGENS AND GLOBAL HEALTH 2019, VOL. 113, NO. 4, 180–190 en_US
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/11532
dc.description.abstract Cutaneous leishmaniasis (CL) caused by Leishmania donovani MON-37 is becoming a major public health problem in Sri Lanka, with 100 new cases per month being reported in endemic regions. Diagnosis of CL is challenging for several reasons. Due to relative specificity and rapidity we propose Fluorescence in Situ Hybridization as a diagnostic tool for CL. The objective was to evaluate the potential of Fluorescence in Situ Hybridization as a diagnostic method for Cutaneous leishmaniasis in Sri Lanka. Literature on current laboratory tests used to diagnose Cutaneous leishmaniasis in Sri Lanka and globally was reviewed. Sri Lankan data were reviewed systematically following the PRISMA guidelines. A narrative of the results is presented. There is currently no gold standard diagnostic method for Cutaneous leishmaniasis. Fluorescence in Situ Hybridization has been previously applied to detect dermal pathologies including those involving infectious agents, and its use to detect the Leishmania parasite in human cutaneous lesions reported in small number of studies, generally with limited numbers of subjects. Advantages of FISH has been specificity, cost and ease-of-use compared to the alternatives. Based on the available literature and our current work, FISH has potential for diagnosing CL and should now be evaluated in larger cohorts in endemic regions. FISH for CL diagnosis could find application in countries such as Sri Lanka, where laboratory facilities may be limited in rural areas where the disease burden is highest. en_US
dc.language.iso en en_US
dc.publisher PATHOGENS AND GLOBAL HEALTH en_US
dc.subject Leishmania; Cutaneous leishmaniasis; Fluorescence in Situ Hybridization; diagnosis; Leishmania donovani; PCR; Slit skin smear; Sri Lanka en_US
dc.title Diagnosing Cutaneous leishmaniasis using Fluorescence in Situ Hybridization: the Sri Lankan Perspective en_US
dc.type Article en_US


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