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Integration of in vitro and in‑silico analysis of Caulerpa racemosa against antioxidant, antidiabetic, and anticancer activities

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dc.contributor.author Dissanayake, I.H.
dc.contributor.author Bandaranayake, U.
dc.contributor.author . Keerthirathna, L.R.
dc.contributor.author Manawadu, C.
dc.contributor.author Silva, R.M.
dc.contributor.author Mohamed, B.
dc.contributor.author Rizwan, A.
dc.contributor.author Peiris, D.C.
dc.date.accessioned 2023-01-25T11:08:33Z
dc.date.available 2023-01-25T11:08:33Z
dc.date.issued 2022
dc.identifier.citation Dissanayake, I.H., et al. (2022). Integration of in vitro and in‑silico analysis of Caulerpa racemosa against antioxidant, antidiabetic, and anticancer activities. Scientifc Reports | (2022) 12:20848 en_US
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/12582
dc.description.abstract Marine algae are found to be excellent in their nutritional and potential therapeutic properties. This study explores the antidiabetic and anticancer potential of fractionated polyphenolic extract of Caulerpa racemosa, green macroalgae. Crude polyphenolic extract (CPE) of C. racemosa and its fractions (n-hexane, ethyl acetate, chloroform, and distilled water) were tested for its total phenol and favonoid contents and antioxidant potential. The ethyl acetate fraction was subjected to gas chromatography/mass spectrometry (GC/MS). The in vitro antidiabetic activity was assessed by alphaamylase, glucosidase inhibition and anti-glycation assays. Also, in-silico studies were conducted to test the binding afnities between caulerpin with alpha-glucosidase enzyme and estrogen receptor (ER) active sites. Each fraction was tested for its in vitroin vitroanticancer activity by CellTiter-Glo and MTT cell proliferation assays. The total phenolic and favonoid contents and the antioxidant potential of the crude extract were observed to be dose dependent. The GC/MS analysis of the ethyl acetate fraction yielded 47 peaks, whereas n-hexadecanoic acid and hexadecanoic acid methyl ester showed the highest compatibility percentages of 99% and 96%, respectively. The CPE exhibited a higher potential in both alpha-amylase inhibitory and anti-glycation activities. The ethyl acetate fraction was more efective against alpha-glucosidase inhibition. Molecular docking revealed a high binding afnity between the alpha-glucosidase enzyme and caulerpin and showed high binding afnity toward caulerpin, with H-bond interactions. The in vitro anticancer analyses revealed that chloroform fraction and CPE exhibited moderate activity on the KAIMRC1 cell line. Also, the CPE exhibited high specifcity compared to the standard drug in anticancer studies. Our fndings evidence the pharmacological potential of the CPE of C. racemosa, and bioactive compounds of the species may be utilized as lead molecules to develop anti-diabetic and anti-cancer drugs. en_US
dc.language.iso en en_US
dc.title Integration of in vitro and in‑silico analysis of Caulerpa racemosa against antioxidant, antidiabetic, and anticancer activities en_US
dc.type Article en_US
dc.identifier.doi https://doi.org/10.1038/s41598-022-24021-y en_US


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