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IN-VITRO INHIBITORY EFFECTS ON ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE AND MODES OF INHIBITION OF Vateria copallifera SEEDS

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dc.contributor.author Mihiranie, M.K.S.
dc.contributor.author Jayasinghe, J.M.J.K.
dc.contributor.author Jayasinghe, C.V.L.
dc.contributor.author Wanasundara, J.P.D.
dc.date.accessioned 2023-03-30T08:33:45Z
dc.date.available 2023-03-30T08:33:45Z
dc.date.issued 2020
dc.identifier.citation Mihiranie, M.K.S., et al. (2020). IN-VITRO INHIBITORY EFFECTS ON ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE AND MODES OF INHIBITION OF Vateria copallifera SEEDS. 7 th International Conference of Multidisciplinary Approaches (iCMA), 2020. en_US
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/12627
dc.description.abstract The objective of present study was to determine the inhibition capacity and modes of inhibition of fresh and debittered Vateria copallifera seed extract against alpha-amylase and alpha-glucosidase enzymatic reactions. In vitro assessment of alpha-amylase and alpha-glucosidase enzyme inhibition capacity was evaluated for 80% ethanol, 80% methanol and aqueous extract of fresh and debittered V. copallifera seeds. Modes of inhibition (Kinetic analysis) were identified using Michaelis Menten and Lineweaver-Burk (double-reciprocal) plots. Results revealed that, 80% ethanol extracts showed the significantly highest alpha-amylase enzyme inhibition capacity (fresh IC50: 36.97±1.28 𝜇g/mL, debittered IC50:98.58±0.55 𝜇g/mL) followed by aqueous extract (fresh IC50: 258.14±12.41 𝜇g/mL, debittered IC50: 410.44±1.24 𝜇g/mL) compared to positive control Acarbose. The highest alpha-glucosidase enzyme inhibition capacity is given by 80% ethanol extract of fresh V. copallifera seeds (IC50: 1469.10±10.01 𝜇g/mL). Kinetic analysis revealed that, 80% ethanol extract inhibited the alpha-amylase competitively although extract displayed a non-competitive mode of inhibition towards alpha-glucosidase. Thus, Vateria copallifera seeds can be considered a good natural resource for the management of Type 2 diabetes with postprandial hyperglycemia due to their traditional acceptability as a healthy food ingredient, availability and low costs with the necessity of further investigations on its active components. en_US
dc.language.iso en en_US
dc.subject V. copallifera, enzyme inhibition activity, Type 2 diabetes mellitus en_US
dc.title IN-VITRO INHIBITORY EFFECTS ON ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE AND MODES OF INHIBITION OF Vateria copallifera SEEDS en_US
dc.type Article en_US


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