dc.contributor.author |
Mihiranie, M.K.S. |
|
dc.contributor.author |
Jayasinghe, J.M.J.K. |
|
dc.contributor.author |
Jayasinghe, C.V.L. |
|
dc.contributor.author |
Wanasundara, J.P.D. |
|
dc.date.accessioned |
2023-03-30T08:33:45Z |
|
dc.date.available |
2023-03-30T08:33:45Z |
|
dc.date.issued |
2020 |
|
dc.identifier.citation |
Mihiranie, M.K.S., et al. (2020). IN-VITRO INHIBITORY EFFECTS ON ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE AND MODES OF INHIBITION OF Vateria copallifera SEEDS. 7 th International Conference of Multidisciplinary Approaches (iCMA), 2020. |
en_US |
dc.identifier.uri |
http://dr.lib.sjp.ac.lk/handle/123456789/12627 |
|
dc.description.abstract |
The objective of present study was to determine the inhibition capacity and modes of inhibition of
fresh and debittered Vateria copallifera seed extract against alpha-amylase and alpha-glucosidase
enzymatic reactions. In vitro assessment of alpha-amylase and alpha-glucosidase enzyme inhibition
capacity was evaluated for 80% ethanol, 80% methanol and aqueous extract of fresh and debittered
V. copallifera seeds. Modes of inhibition (Kinetic analysis) were identified using Michaelis Menten
and Lineweaver-Burk (double-reciprocal) plots. Results revealed that, 80% ethanol extracts showed
the significantly highest alpha-amylase enzyme inhibition capacity (fresh IC50: 36.97±1.28
𝜇g/mL, debittered IC50:98.58±0.55 𝜇g/mL) followed by aqueous extract (fresh IC50: 258.14±12.41
𝜇g/mL, debittered IC50: 410.44±1.24 𝜇g/mL) compared to positive control Acarbose. The highest
alpha-glucosidase enzyme inhibition capacity is given by 80% ethanol extract of fresh V. copallifera
seeds (IC50: 1469.10±10.01 𝜇g/mL). Kinetic analysis revealed that, 80% ethanol extract inhibited
the alpha-amylase competitively although extract displayed a non-competitive mode of inhibition
towards alpha-glucosidase. Thus, Vateria copallifera seeds can be considered a good natural
resource for the management of Type 2 diabetes with postprandial hyperglycemia due to their
traditional acceptability as a healthy food ingredient, availability and low costs with the necessity
of further investigations on its active components. |
en_US |
dc.language.iso |
en |
en_US |
dc.subject |
V. copallifera, enzyme inhibition activity, Type 2 diabetes mellitus |
en_US |
dc.title |
IN-VITRO INHIBITORY EFFECTS ON ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE AND MODES OF INHIBITION OF Vateria copallifera SEEDS |
en_US |
dc.type |
Article |
en_US |