dc.contributor.author |
Pathmanathan, N. |
|
dc.contributor.author |
Deshappriya, N. |
|
dc.contributor.author |
Manamgoda, D.S. |
|
dc.contributor.author |
Sandamali, T.G.I. |
|
dc.date.accessioned |
2023-04-03T08:24:34Z |
|
dc.date.available |
2023-04-03T08:24:34Z |
|
dc.date.issued |
2022 |
|
dc.identifier.citation |
Pathmanathan, N. , et al. (2022). Diversity of the Endophytic Fungal spp. in Selected Rice (Oryza sativa L) Varieties of Sri Lanka and their Hydrolytic Enzyme Producing Abilities. 26th International Forestry and Environment Symposium 2022 o f the Department ofForestry and Environmental Science, University o f Sri Jayewardenepura, Sri Lanka |
en_US |
dc.identifier.uri |
http://dr.lib.sjp.ac.lk/handle/123456789/12676 |
|
dc.description.abstract |
Rice (Oryza sativa L.) is the staple food in Sri Lanka and with increases in population, the demand
for rice has also increased requiring higher yields. The use of endophytic fungal (EF) assemblages
has been successful in increasing the yields in a number of crops including rice. However, the existent
knowledge on the diversity among the species of EF assemblages associated with different rice
varieties is hardly sufficient for this purpose. Therefore, this study was aimed at identifying and
assessing the diversity of EF present in three newly improved rice varieties At 362, Bg 352, Bw 367
and one traditional variety i.e. Suwandel grown in different geographical locations/climatic zones of
Sri Lanka. Healthy plant samples of each rice variety was collected during the Maha and Yala seasons
in 2018/2019 from Anuradhapura, Kurunegala, Gampaha and Kalutara districts in Sri Lanka.
Endophytic fungi were isolated from leaves, stems and roots of the four rice varieties using previously
optimized protocols. Identifications of the isolated fungal spp. was carried out using morphological
and molecular characteristics. Species were identified by PCR amplification of the Internal
Transcriber Spacer (ITS) regions and comparing their sequences with those of well characterized/type
strains in the National Centre for Biotechnology Information (NCBI) database. 1,920 plant segments
used for isolations yielded 26 fungal genera and 39 fungal spp. The most frequently isolated and
dominant species among all rice varieties were Microdochium fisheri, Dendryphiella sp and
Penicillium oxalicum. Species diversity was analyzed using Shannon Wiener’s (H’) and Simpson’s
dominance (1-D) indices and a high diversity of different fungal spp. were observed in Bg 352
collected from Kalutara during the Yala season. As EF have been reported to produce extra cellular
enzymes as a means of showing mycoparasitic activity, selected EF isolates were screened in enriched
media for production of Chitinase and Protease enzymes using standard plate assays and Glucanase
enzyme production was analyzed by Dinitro Salicylic Acid assay. Out of the tested isolates, Rhizopus
microsporus produced a significantly high (p<0.05) level of chitinase, while Aspergillus fischeri
showed a significantly high (p<0.05) protease production. Penicillium oxalicum showed a
significantly high (p<0.05) glucanase production. Thus, findings of this study postulate rich EF
assemblages in Sri Lankan rice varieties which could potentially be a foundation for studies on novel
models of rice-fungal mutualism. Rice endophytes also produce extra-cellular hydrolytic enzymes
that may contribute towards controlling rice pathogens. |
en_US |
dc.language.iso |
en |
en_US |
dc.subject |
Endophytes, Fungal diversity, Rice varieties, ITS region |
en_US |
dc.title |
Diversity of the Endophytic Fungal spp. in Selected Rice (Oryza sativa L) Varieties of Sri Lanka and their Hydrolytic Enzyme Producing Abilities |
en_US |
dc.type |
Article |
en_US |