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A Preliminary Study on Application of Phage-indicator Model in Evaluation of Antiviral Drugs

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dc.contributor.author Malalasekara, A.M.A.L.R.
dc.contributor.author Jayaratne, D.L.
dc.date.accessioned 2015-12-01T05:59:12Z
dc.date.available 2015-12-01T05:59:12Z
dc.date.issued 2015-12-01T05:59:12Z
dc.identifier.citation Malalasekara, A.M.A.L.R., & Jayaratne, D.L. (2015). A Preliminary Study on Application of Phage-indicator Model in Evaluation of Antiviral Drugs. Proceedings of the 71st Annual Sessions of Sri Lanka Association for the Advancement of Science (Part I), 57.
dc.identifier.issn 13910248
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/2006
dc.description.abstract Due to newly emerging viral diseases and the building of rapid resistance among targeted viruses, the continuous search for useful and novel antiviral compounds has become important. Hence, a study was carried to develop a method for the preliminary screening of antiviral drugs and identification of their targeted stage of the viral replication cycle. A model system of bacteriophage and susceptible Escherichia coli, isolated together from a sewage effluent was used for the screening process. Two plant extracts, each from Carica papaya leaves and Psidium guajava leaves and two plant based products each “Sudarshana Churnaya”, an aurvedic drug and black tea produced from Camellia sinensis leaves were screened using two approaches developed based on plaque reduction assay to detect their effect on different stages of the phage replication cycle. In the first approach, the purified virus suspensions pre-incubated with filter sterilized herbal extracts were used to detect the antiviral effects of herbal extracts on adsorption and penetration steps. For the second approach, the host bacterium pre-incubated in filter sterilized herbal extracts were used as the indicator host to detect the effects of herbal extracts on intracellular replication steps of the virus replication cycle. Screening revealed that black tea has the ability to inhibit viral propagation by preventing phage attachment to their host and Carica papaya leaf extract showed an ability to inhibit intracellular stage(s) of the bacteriophage’s replicative cycle. The study provides evidence for the availability of antiviral compounds in plant extracts and plant based products which are used in routine life and traditional healing methods. The results also indicate that different herbal extracts apply different modes of action to prevent the propagation of the targeted virus. en_US
dc.language.iso en en_US
dc.subject Antiviral drugs en_US
dc.subject bacteriophages en_US
dc.subject phage replication en_US
dc.subject plaque reduction assay en_US
dc.title A Preliminary Study on Application of Phage-indicator Model in Evaluation of Antiviral Drugs en_US
dc.type Article en_US
dc.date.published 2015


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