Attached
Carbohydrates as a group are of high importance, considered
from both the practical and theoretical aspects. The different types
of polysaccharides linked by glycosidic bonds, account for the
different solubility, reactivity and appearance of the vast range of
carbohydrates, which occur in living organisms.
The behaviour of partially methylated sugars and methylated
glycosides were studied in hplc using different columns and different
solvent systemso The information thus obtained was used to investigate
the structures of unknown carbohydrateso The results sllow that hplc is
useful in the methylation analysis of polysaccharides, and for the
preparation of reference compounds.
Study of the formation of methyl glycosides showed that hplc
is useful for the preparation of many methyl glycosideso Rates of
glycosidation could be followed and op~imum conditions determined for
the formation of a particular glycoside. The method is also applicable
to the synthesis of the methyl glycosides of disaccharides, as shown by
the isolation of the methyl glycosides of maltose. The glycoside work
was extended to the compositional analysis of polysaccharideso
Glycosides formed during methanolysis of polysaccharides were separated
and quantitatively estimated.
Successful separation of oligosaccharide series up to DP 8-9
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was achieved. Oligomers of the same DP but from different structural
series e.g. the maltose and isomaltose series, had different retention
times, showing that the separation mechanism operating was structure
dependento
