Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m7GpppG

dc.contributor.author	Gunawardana, D.
dc.contributor.author	Domashevskiy, A.V.
dc.contributor.author	Gayler, K.R.
dc.contributor.author	Goss, D.J.
dc.date.accessioned	2017-04-03T05:27:36Z
dc.date.available	2017-04-03T05:27:36Z
dc.date.issued	2015
dc.identifier.citation	Gunawardana, D., Domashevskiy, A.V., Gayler K.R., & Goss, D.J. (2015). Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m7GpppG. Translation, 3(1). doi: 10.4161/21690731.2014.988538	en_US, si_LK
dc.identifier.uri	http://dr.lib.sjp.ac.lk/handle/123456789/4728
dc.description.abstract	A method has been developed for synthesising fluorescentiy labeled capped mRNA. The method incorporates a single fluorescent molecule as part of the 5'-mRNA or oligonucleotide cap site. The fluorescent molecule, Ant-m7GTP is specifically incorporated into the cap site to yield Ant-m7GpppG-capped mRNA or oligonucleotide. Efficient capping was observed with 60-100% of the RNA transcripts capped with the fluorescent molecule. The Ant-m7G derivative, which has been previously shown to interact with the eukaryotic cap binding protein elF4E, is shown in this paper to be a substrate for the Vaccinia capping enzyme and the DCP2 decapping enzyme from Arabidopsis. Further, the Antm7GTP- capped RNA is readily translated. This Ant-m7GTP-capped RNA provides an important tool for monitoring capping reactions, translation, and biophysical studies.	en_US, si_LK
dc.language.iso	en	en_US, si_LK
dc.publisher	Taylor & Francis, London	en_US, si_LK
dc.subject	capped mRNA	en_US, si_LK
dc.subject	decapping assay	en_US, si_LK
dc.subject	Fluorescence	en_US, si_LK
dc.subject	imaging	en_US, si_LK
dc.subject	RNA capping	en_US, si_LK
dc.title	Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m7GpppG	en_US, si_LK
dc.type	Article	en_US, si_LK