Maximizing Proliferation of Lactobacillus Acidophilus and Bifidobacterium animalis Sub Sp. lactis BB -12 In Vitro by Integrating Different Sources of Prebiotics

Abstract

Felicitating probiotics by prebiotics attribute to harvest the promised health benefits from probiotics. This study aims to produce an integrated prebiotic combination which consists of three different sources of plant extracts, capable of significantly increasing the growth of two probiotic species, Lactobacillus acidophilus and Bifidobacterium animalis subsp. Iactis BB-12 separately in vitro and thereby optimizing health benefits. The three different sources of prebiotics considered in this experiment were fibre, polyphenolic extracts and non-digestibie polysaccharide extracts which were isolated from Musa sp pseudostem, Sesbania grandiflora flowers and Artocarpus heterophylius seeds. Eight treatments were designed by integrating three sources of prebiotics at two different levels i.e; fibre (0.2% and 2%), polyphenolic extracts (0.2% and 0.6%) and non-digestible polysaccharide extracts (0.2% and 1.2%). Three treatments were designed by including only one ingredient at a time i.e.; fibre 0.2%, polyphenolic extracts 0.2% and non-digestible polysaccharide extracts 0.2%.The effect of all eleven treatments were assessed against the growth promoting ability on L. acidophilus and Bifidobacterium animalis subsp. Iactis-BB12 in liquid culture media and analysed using Minitab-16 Statistical software. The treatment which consisted 2% fibre, 0.2% polyphenolic extract and 0.2% non-digestible polysaccharide extract was able to produce a peak proliferation for Lactobacillus acidophilus, while the treatment consisting 2% fibre, 0.6% polyphenolic extracts and 0.2% non-digestible polysaccharide extracts resulted in the highest proliferation for Bifidobacterium animalis subsp. Iactis BB-12 in vitro which were significantly different (p<0.05) from rest of the treatments. Test probiotic organisms supplemented with integrated prebiotic combinations, showed higher growth rate than, when grown with treatments with one prebiotic ingredient at a time. Musa sp fibre isolate showed higher potential of causing significant increase of Loctobacillus acidophilus biomass, while polyphenolic extracts of Sesbania grandiflora flower imposed significant effect on increasing the growth of Bifidobacterium animalis subsp. /actis-BB12 when considered as separate entities in liquid culture media.