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The prevalence of Dengue has dramatically increased in the tropics in recent decades. However, the
present therapeutic strategies for disease prevention still remains controversial. Therefore, the purpose
of this study is to identify a possible molecular target for genome editing which could eradicate the
virus.. Availability of the genome sequence data and the sophisticated bioinformatics tools are
versatile platforms to investigate the novel target for genome editing. The study is based on the
genomic sequence data of four dengue virus serotypes obtained from the National Center for
Bioteclinology Information, sequence similarity search using Basic Local Alignment Tool (BLAST)
and the introduction of a novel gene editing technique as a way to eradicate the virus. The nucleotide
BLAST search was performed against the nucleotide collection database for different regions of the
genomes of all four serotypes. The nucleotide sequences which showed significant similarity with the
database sequences were discarded. The nucleotide sequence 3’
gaacateatgtggaagcaaatatcaaatgaattaaaccacatcttacttgaaaatgacatgaaatttacagtggtcgtaggagacgttagtggaatctt
ggcccaaggaaagaaaatgattaggccacaacccatggaacacaaatactcgtggaaaagctggggaaaagccaaaatcataggagcagatgt
acagaataccaccttcat 5’ was proposed as the drug target for dengue virus 1, the nucleotide sequence 3’
tgtagctccgtcgtggggacgtaaaacctgggaggctgcaaactgtggaagctgtacgcacggtgtagcagactagcggttagaggagacccctc
ccatgacacaacgcagcagcggggcccgagcactgagggaagctgtacctccttgcaaaggactagaggttagaggagaccccccgcaaataa
aa 5’ for dengue virus 3 and the nucleotide sequence 3’
aagacattccgcagtgggaaccatctaagggatggaaaaactggcaagaggttcctttttgctcccaccactttcacaagatctttatgaaggatggc
cgctcactagttgttccatgtagaaaccaggatgaactgatagggagagccagaatctcgcagggagctggatggagcttaagagaaacagcctg
cctgggcaaagcttacg 5’ for dengue virus 4 respectively. All above sequences code for polyprotein
precursors, structural and non-structural proteins and for the translation factors and do not have any
sequence similarity to human genomic DNA at expect threshold valtie of 100. The study also suggests
that the sequence specific designer nucleases or the programmed nucleases which functions to precise
edition of the above target sequences as a method to eradicate Dengue. Eradication of the virus would
have less environmental effects than the present methods of controlling the mosquito, which acts as
the vector
