Attached
Hydroxycitric acid (HCA), both free acid and
lactone forms is present in the fruit rind, of Garcinia
species and it is known to help to prevent obesity. HCA
is isolated from dehydrated Garcinia cambogia fruit rind
at 600C, by the defined most efficient extraction method
of refluxing for 5 hours in soxhelt apparatus using 99%
ethanol at 60°C. acid base titration with 0.5M KOH(eih)
determined The total acid content of the extracted
solutions was 0.50 - 0.51g g per 1 g. most stabilized
precipitate of the salt of HCA was formed with 0.05M
ethanolic KOH and it was able to crystallized into a dry
form by drying in a rotary vacuum evaporator, below
40"C. The formed dry precipitate was further preserved
by incorporating anti caking agent at the ratio of 1:3
(vacuum dried potassium salt of HCA: corn starch). The
amount of HCA in the isolated precipitate and the
extract was analyzed by HPLC and UV-Visible
Spectroscopy and obtained graph was compared with
standard potassium salt of HCA. T h e ). max was obtained
at 208 nm when the solution of isolated HCA was scanned
between 200 - 400 nm. Content of HCA present in the
plant extract has been found to be in the range of 42 -
44% by using HPLC with 0.01M hydrochloric acid as
mobile phase with a flow rate of 0.5ml/min using UV
detection at 208 nm. Retention time of HCA was
observed at 4.535 min in selected mobile phase. Pellets
were obtained by mixing aqua edible gum solution with
the preserved powder of HCA salt in I: 4 ratio and
drying at 60°C for 12 hours after molding in to pellet
shape.
