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Removal of Microcystin-Lr Using Cellular Extracts of Bacillus Cereus

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dc.contributor.author Pathmalal, M. M.
dc.date.accessioned 2017-10-23T05:32:23Z
dc.date.available 2017-10-23T05:32:23Z
dc.date.issued 2017
dc.identifier.citation M.M. Pathmalal, (2017), "Removal of Microcystin-Lr Using Cellular Extracts of Bacillus Cereus", KDU Journal of Multidiciplinary Studies (KJMS), Vol. 1, Iss: 1, PP. 1 - 9 en_US, si_LK
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/6020
dc.description.abstract Microcystin –LR (MC-LR) is considered to be the most dominant type of cyanobacterial toxins present in water bodies. The present study focuses on using cellular extracts of Bacillus cereus in removal of MC-LR in water. Bacterial cell extracts were prepared using overnight grown fresh cultures of B. cereus which was previously recorded as a potent MC-LR degrading bacterium. Bacterial cell disruption was performed by bead beating on a micro-mini bead beater. Cell debris was removed by centrifugation at 13000 rpm, 20 min. Subsequently, a series of concentrations of cellular extract (100%, 75%, 50% and 25%) was prepared. These cell extracts were separately incubated at 280C with 100μg ml-1 of MC-LR for a period of 4 days. 1ml aliquots were removed at 24 hour intervals for four days and frozen at (-20) 0C. Then frozen samples were freeze-dried and subjected to Photo diode array- High Performance Liquid Chromatography (PDA-HPLC) analysis to detect the remaining MC-LR concentrations of the samples. At the end of fourth day, 81.1 μgml-1 of MC-LR was removed when 100% of cell extract was used. When 75 % of cell extract was used, 77.6 μgml-1 of MC-LR removal was evident at the end of fourth day. Whereas when 50 % and 25% of cell extract were used only 40.7 μgml-1 and 25.7 μgml-1 of MC-LR removal was detected respectively. The results of the present study indicate that bacterial cell extracts of B. cereus has the ability to remove MC-LR by an enzyme mediated mechanism. en_US, si_LK
dc.language.iso en_US en_US, si_LK
dc.subject Microcystin-LR en_US, si_LK
dc.subject Bacillus cereus en_US, si_LK
dc.subject cellular extracts en_US, si_LK
dc.subject PDA-HPLC en_US, si_LK
dc.title Removal of Microcystin-Lr Using Cellular Extracts of Bacillus Cereus en_US, si_LK
dc.type Article en_US, si_LK


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