dc.contributor.author |
Pathmalal, M. M. |
|
dc.date.accessioned |
2017-10-23T05:32:23Z |
|
dc.date.available |
2017-10-23T05:32:23Z |
|
dc.date.issued |
2017 |
|
dc.identifier.citation |
M.M. Pathmalal, (2017), "Removal of Microcystin-Lr Using Cellular Extracts of Bacillus Cereus", KDU Journal of Multidiciplinary Studies (KJMS), Vol. 1, Iss: 1, PP. 1 - 9 |
en_US, si_LK |
dc.identifier.uri |
http://dr.lib.sjp.ac.lk/handle/123456789/6020 |
|
dc.description.abstract |
Microcystin –LR (MC-LR) is considered to be the most dominant type of cyanobacterial toxins present in water
bodies. The present study focuses on using cellular extracts of Bacillus cereus in removal of MC-LR in water.
Bacterial cell extracts were prepared using overnight grown fresh cultures of B. cereus which was previously
recorded as a potent MC-LR degrading bacterium. Bacterial cell disruption was performed by bead beating on
a micro-mini bead beater. Cell debris was removed by centrifugation at 13000 rpm, 20 min. Subsequently, a
series of concentrations of cellular extract (100%, 75%, 50% and 25%) was prepared. These cell extracts were
separately incubated at 280C with 100μg ml-1 of MC-LR for a period of 4 days. 1ml aliquots were removed at
24 hour intervals for four days and frozen at (-20) 0C. Then frozen samples were freeze-dried and subjected to
Photo diode array- High Performance Liquid Chromatography (PDA-HPLC) analysis to detect the remaining
MC-LR concentrations of the samples. At the end of fourth day, 81.1 μgml-1 of MC-LR was removed when 100%
of cell extract was used. When 75 % of cell extract was used, 77.6 μgml-1 of MC-LR removal was evident at the
end of fourth day. Whereas when 50 % and 25% of cell extract were used only 40.7 μgml-1 and 25.7 μgml-1 of
MC-LR removal was detected respectively. The results of the present study indicate that bacterial cell extracts of
B. cereus has the ability to remove MC-LR by an enzyme mediated mechanism. |
en_US, si_LK |
dc.language.iso |
en_US |
en_US, si_LK |
dc.subject |
Microcystin-LR |
en_US, si_LK |
dc.subject |
Bacillus cereus |
en_US, si_LK |
dc.subject |
cellular extracts |
en_US, si_LK |
dc.subject |
PDA-HPLC |
en_US, si_LK |
dc.title |
Removal of Microcystin-Lr Using Cellular Extracts of Bacillus Cereus |
en_US, si_LK |
dc.type |
Article |
en_US, si_LK |