Attached
The growth stimulatory effect produced by combining different sources of prebiotics i.e; Fiber isolates
from Musa sp pseudostem, polyphenol extracts from Sesbania grandiflora flower petal and non-digestible
polysaccharide extracts from Artocarpus heterophyllus seed were assessed against probiotic organisms,
Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis BB-12 in vitro in liquid cultures. Different
combinations were formulated by integrating the three sources of prebiotics at two different levels i.e; fibre (0.2%
and 2%), polyphenol extracts (0.2% and 0.6%) and non-digestible polysaccharide extracts (0.2% and 1.2%) to obtain
eight treatments. The formulation which consisted 2% fibre, 0.2% polyphenol and 0.2% non-digestible
polysaccharide was able to promote significant biomass increment in Lactobacillus acidophilus, while the treatment
consisting 2% fibre, 0.6% polyphenol and 0.2% non-digestible polysaccharide demonstrated highest proliferation for
Bifidobacterium animalis subsp. lactis BB-12 in vitro which were statistically different (p<0.05) than other
formulations.
