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Bioactivity and volatile profiling of Azadirachta indica leaves for the suppression of Maize Weevil, Sitphilus zeamais (Motsch.) infestations (FP1017)

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dc.contributor.author Perera, A.G.W.U.
dc.contributor.author Karunaratne, M.M.S.C.
dc.contributor.author Chinthaka, S.D.M.
dc.date.accessioned 2017-10-26T05:23:16Z
dc.date.available 2017-10-26T05:23:16Z
dc.date.issued 2016
dc.identifier.citation Perera, A.G.W.U., Karunaratne, M.M.S.C., Chinthaka, S.D.M. (2016). "Bioactivity and volatile profiling of Azadirachta indica leaves for the suppression of Maize Weevil, Sitphilus zeamais (Motsch.) infestations (FP1017)", Proceedings of National Conference on Green Practices – 14th July 2016 Water's Edge, Battaramulla., p. 30 en_US, si_LK
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/6157
dc.description.abstract Attached en_US, si_LK
dc.description.abstract Neem (Azadirachta indica A. juss), is known to possess a wide range of pharmacological properties and is thus commercially exploitable. Apart from its medicinal potential, a considerable progress has been achieved regarding biological potential and composition of volatile fraction of the leaves which is an ever-increasing interest to th e scientific community. During this study, biological phenom ena and for the first tim e, volatile composition of A. indica leaves w ere examined in the m anagem ent of Sitophilus zeamais on stored maize. Efficacy of neem leaves was tested by admixing leaf powders with maize grains at 5 doses (3.33%, 10%, 16.67% , 23.33% , and 33.33% w /w ). Using m odified cup-bioassays, repellent effect was assessed after an hour of weevil introduction, w hile contact and fum igation toxic potential of leaf powders w ere evaluated at 24 hour intervals up to 10 days of weevil exposure. Volatile profile of A. indica leaves was characterized by employing headspace-solid-phase micro extraction coupled with gas chromatography-mass spectrom etry (HS-SPME/GC-MS). Over 60% weevil repellency was recorded at doses above 23.33% , whereas 100% and 67% contact and fum igation mortalities w ere observed respectively, 9 days after treatm en t at the dose of 33.33% . Four volatile compounds w ere identified accounting for 99.98% of the total detected constituents in three distinct chemical classes (Sesquiterpenoid, aldehyde and halogenated aliphatic hydrocarbon). G am m a.-elem ene (54.96% ) and 2-hexenal (33.33% ) w ere the m ajor constituents of neem leaf volatiles, followed by m ethylene chloride (6.05%) and caryophyllene (5.65% ) which are responsible for varied biological activities observed. Odor impact of th e bioassay-guided study clearly implies th a t A. indica leaves can be harnessed against S. zeamais infestations.
dc.language.iso en_US en_US, si_LK
dc.publisher Proceedings of National Conference on Green Practices – 14th July 2016 Water's Edge, Battaramulla. en_US, si_LK
dc.title Bioactivity and volatile profiling of Azadirachta indica leaves for the suppression of Maize Weevil, Sitphilus zeamais (Motsch.) infestations (FP1017) en_US, si_LK
dc.type Article en_US, si_LK


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