Synthesis, characterization and BSA binding studies of novel zinc ferene complexes

Abstract

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Most triazine derivatives show various biological applications. In this study two Zn(II) complexes were synthesized by using the commercially available ligand, ferene [3-(2-pyridyl-5,6-di(2-furyl)-1,2,4- triazine- p, p'disulfonicacid disodium saltJ.The mono complex, Zn(ferene)Cl2(l), was synthesized by reacting starting material (ZnCl2: Ferene) in a 1:1 ratio and the bis complex, Zn(ferene)2Cl2(2) was synthesized by reacting starting material (ZnCl2: Ferene) in a 1: 2 ratio, respectively (Scheme 1). Both the synthesized complexes are highly soluble in water, making them good candidates for bio assays. The synthesized complexes have been characterized by spectroscopic techniques. According to the UV- Vis spectra for all the complexes (1 and 2) a bathochromic shift has been observed (Figure 1). Also FTIR data provide evidence that Zn-N bonds are formed via triazine and pyridine N atoms. Accordingly the stretching frequency of N=N and C=N bonds of complexes have been shifted to the low frequency range in comparison with the free ligand. 'H NMR spectra were obtained for both complexes and confirmed their purity. Thereafter, the binding studies of mononuclear zinc(II) complexes (1 and 2) and ferene ligand with bovine serum albumin (BSA) have been investigated by UV-Vis absorption spectroscopy. With increasing the concentrations of ferene and complexes (1) and (2) the maximum absorbance was increased without any shift in position of die curve. These results indicate the interaction with BSA changing the polarity of the microenvironment around tryptophan and tyrosine amino acid residue. The binding constants (Kb) for ferene, complexes 1 and 2 with BSA were calculated as 3.09* 104M ‘, 12.30 * 104M'‘ and 16.84 x 104M ‘, respectively.