Attached
– The resistance among various bacterial species to different antibiotics has emerged as a cause of
public health threat in all over the world at a terrifying rate. The present study was carried out to assess
the antibiotics resistance of 378 bacteria strain isolated from hospital effluent water in Sri Lanka. Antibioticresistant bacteria were identied using 16s rRNA sequencing and the Minimum Inhibition Concentration
(MIC) was determined using agar dilution method. Multiple Antibiotic Resistance (MAR) was determined
using 96 well plate method in order to calculate the MAR index. Descending trend of bacterial resistance
against five antibiotics; AMX ( 28%), AMP (21%) SUF/TRI (9%) and SDI (9%) were detected. Bacillus sp.
(44%) was the most frequently isolated gram positive bacteria where Staphylococcus sp. (23%), Micrococcus
sp. (15%), Streptococcus sp. (10%), Lactobacillus sp. (8%) and Streptomyces sp. (1%) showed decending order.
Among the isolated gram negative bacteria Enterobacter sp. (31%) and E. coli (31%) were the dominant
bacteria strains whereas Acinetobacter sp. (19%), Pseudomonas aerugenosa (8%), Klebsiella pneumonia (6%),
Moraxella sp. (3%), Aeromonas hydrophila (1%) were recorded in low densities. MIC limits of resistant isolates
against to the tested antibiotics were ranged between 60 – 660 µg/ml. 11% of bacteria was susceptible to all
the tested antibiotics; 9% of bacteria were resistant for one antibiotic, 12% were for 2 antibiotics and 68%
were for 5 or more antibiotics. The results of the study revealed that release of antibiotics into the
environment will lead to an emerge antibiotic resistant bacteria and it compromise effectiveness of
antibacterial therapy; since the infectious organisms become resistant against more antibiotics.
