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Herbal beverages with desirable sensory attributes are an ideal way to offer consumers with phytochemicals having specific health
promoting functionalities. Syzygium cumini bark decoction is used in treating diabetes mellitus in Ayurveda medicine. This work
attempted to prove the presence of antidiabetic compounds in the S. cumini decoction and the ready to serve (RTS) herbal drink
developed using the decoction. Activity guided fractionation of the decoction of the S. cumini was carried out by sequential
extraction with organic solvents of different polarities. Thin Layer Chromatography (TLC) with authentic compounds and HPLC
were performed for identification and confirmation of the compounds in the decoction and the RTS herbal drink. Presence of gallic
and ellagic acids in the decoction and RTS herbal drink was proven and confirmed with HPLC. The LC UV-VIS spectra of the two
compounds were identical with the corresponding spectra of the library. Further, gallic acid and umbelliferone were determined as
the active compounds in the decoction by TLC and were confirmed by cochromatography with authentic compounds. The present
investigation confirmed the presence of gallic acid, ellagic acid, and umbelliferone which are proven to have antidiabetic activities
in the decoction and the RTS herbal drink prepared with the decoction.