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Removal of Ciprofloxacin (CIP) by Bacteria Isolated from Hospital Effluent Water and Identification of Degradation Pathways

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dc.contributor.author Liyanage, G.Y.
dc.contributor.author Manage, P.M.
dc.date.accessioned 2018-06-04T15:31:33Z
dc.date.available 2018-06-04T15:31:33Z
dc.date.issued 2018
dc.identifier.citation Liyanage, G.Y., Manage, P.M. (2018). "Removal of Ciprofloxacin (CIP) by Bacteria Isolated from Hospital Effluent Water and Identification of Degradation Pathways",International Journal of Medical, Pharmacy and Drug Research (IJMPD), Vol. 02 (3), pp. 37-47 en_US, si_LK
dc.identifier.issn 2456-8015
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/6994
dc.description.abstract Attached en_US, si_LK
dc.description.abstract Most antibiotics are metabolized incompletely by patients after administration and enter the municipal sewage with the patients’ excretion. Therefore, studies on the biodegradability of some clinically important drugs can be taken as a very first step of an environmental risk assessment. The present study reports the biodegradation of CIP by Lactobacillus gesseri, Enterobacter sp., Bacillus sp., Bacillus subtilius and Micrococcus luteus which were isolated as CIP resistance, non pathogenic bacteria. The presence of antibioticresistant bacteria was identified using the 16s rRNA sequencing. A 0.5ml of overnight starved bacterial suspensions was introduced into medium containing CIP at 5 ppm. Triplicate samples were incubated at 280 C with shaking at 100ppm. A 0.5 ml of subsamples was removed at 2 days interval for a period of 14 days. Samples were subjected to High Performance Liquid Chromatography (HPLC) analysis. Fourier Transform Infrared Spectroscopy (FTIR) analyses were carried out for each sample at the end of the 14 days to find structures of by-products. Complete degradation of CIP by L. gasserri was detected at the end of 14 days of incubation with average degradation rate of 0.182 ±0.15µg /day. Descending degradation rates were followed by Enterobacter sp. (0.75 ±0.03 d-1 ) and Bacillus sp. (0.41±0.02d-1 ) at 8 and 6 days respectively. However, clear cut degradation of CIP was not detected for B.subtilis and Micrococcus luteus respectively. Further, FTIR spectrum revealed that incubation of L. gesseri, Enterobacter sp. and Bacillus sp., changed the piperazine ring and quinolone part in the CIP structure while degradation occurred
dc.language.iso en_US en_US, si_LK
dc.publisher International Journal of Medical, Pharmacy and Drug Research (IJMPD) en_US, si_LK
dc.subject Ciprofloxacin en_US, si_LK
dc.subject Degradation en_US, si_LK
dc.subject Fourier Transform Infrared Spectroscopy (FTIR) en_US, si_LK
dc.subject High Performance Liquid Chromatography (HPLC) en_US, si_LK
dc.subject Lactobacillus gesseri en_US, si_LK
dc.title Removal of Ciprofloxacin (CIP) by Bacteria Isolated from Hospital Effluent Water and Identification of Degradation Pathways en_US, si_LK
dc.type Article en_US, si_LK


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