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Removal of microcystin-LR using ceelular extracts of Bacillus cereus

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dc.contributor.author Idroos, F. Sumaiya
dc.contributor.author Manage, Pathmalal M.
dc.date.accessioned 2018-11-12T07:31:36Z
dc.date.available 2018-11-12T07:31:36Z
dc.date.issued 2017
dc.identifier.citation Idroos, F. Sumaiya , Manage, Pathmalal M. (2017). "Removal of microcystin-LR using ceelular extracts of Bacillus cereus" , KDU Journal of Multidiciplinary Studies (KJMS) Vol. 1.(01), pp. 5-9 en_US
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/7356
dc.description.abstract attached en_US
dc.description.abstract Microcystin –LR (MC-LR) is considered to be the most dominant type of cyanobacterial toxins present in water bodies. The present study focuses on using cellular extracts of Bacillus cereus in removal of MC-LR in water. Bacterial cell extracts were prepared using overnight grown fresh cultures of B. cereus which was previously recorded as a potent MC-LR degrading bacterium. Bacterial cell disruption was performed by bead beating on a micro-mini bead beater. Cell debris was removed by centrifugation at 13000 rpm, 20 min. Subsequently, a series of concentrations of cellular extract (100%, 75%, 50% and 25%) was prepared. These cell extracts were separately incubated at 280C with 100µg ml-1 of MC-LR for a period of 4 days. 1ml aliquots were removed at 24 hour intervals for four days and frozen at (-20) 0C. Then frozen samples were freeze-dried and subjected to Photo diode array- High Performance Liquid Chromatography (PDA-HPLC) analysis to detect the remaining MC-LR concentrations of the samples. At the end of fourth day, 81.1 µgml-1 of MC-LR was removed when 100% of cell extract was used. When 75 % of cell extract was used, 77.6 µgml-1 of MC-LR removal was evident at the end of fourth day. Whereas when 50 % and 25% of cell extract were used only 40.7 µgml-1 and 25.7 µgml-1 of MC-LR removal was detected respectively. The results of the present study indicate that bacterial cell extracts of B. cereus has the ability to remove MC-LR by an enzyme mediated mechanism.
dc.language.iso en en_US
dc.subject Microcystin-LR en_US
dc.subject Bacillus cereus en_US
dc.subject Cellular extracts en_US
dc.subject PDA-HPLC en_US
dc.title Removal of microcystin-LR using ceelular extracts of Bacillus cereus en_US
dc.type Article en_US


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