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A field study was conducted on two texturally different soils to determine the influences of biosolids
application on selected soil chemical properties and carbon dioxide fluxes. Two sites, located in Manildra
(clay loam) and Grenfell (sandy loam), in Australia, were treated at a single level of70 Mg ha-I biosolids.
Soil samples were analyzed for SOC fractions, including total organic carbon (TOC), labile, and non-labile
carbon contents. The natural abundances of soil 0 Be and 015N were measured as isotopic tracers to
fingerprint carbon derived from biosolids. An automated soil respirometer was used to measure in-situ
diurnal C02 fluxes, soil moisture, and temperature. Application of biosolids increased the surface (0
-15 em) soil TOe by > 45% at both sites, which was attributed to the direct contribution from residual
carbon in the biosolids and also from the increased biomass production. At both sites application of
biosolids increased the non-labile carbon fraction that is stable against microbial decomposition, which
indicated the soil carbon sequestration potential of biosolids. Soils amended with biosolids showed
depleted o13C, and enriched olsN indicating the accumulation ofbiosolids residual carbon in soils. The in
