Attached
Pineapple (Ananas comosus L) is a popular fruit among Sri Lankans. The peel (41 % of whole
fruit) is usually discarded during its processing, and high amount of sugars in the attached flesh of
pineapple peel favorable substrate for fermentation process. The present study was focused in
production of pineapple peel vinegar in two modes i.e., development of condiment (3 % acetic acid)
with spice addition and development of vinegar (4% acetic acid) for commercialization. Mature
pineapple (Anans comosus L) peel (Mauritius) obtained from a fruit processing industry was used
for the study. Spice added condiment was produced by two-step process, i.e., alcoholic and acetic
acid fermentation using pineapple peel. Alcoholic fermentation was done in both 25± 1°C and room
temperature (30±I°C) until the alcohol content reached 9% (w/v). Acetic acid fermentation was
carried out with unpasteurized vinegar (Acetobacteraceti) with continuous aeration until acid level
reached 2% and 3% (in separate batches) followed by pasteurization (72°C, 15 sec) and filtration
using muslin cloth. Crushed (0.5-l.0 mm) cardamom and cinnamon were added separately at 1.5
and 2 % (w/v) levels to the above condiments and were aged at room temperature for two weeks.
Spiced condiment were obtained by filtration and subjected to sensory evaluation. The best
temperature found for alcohol fermentation was 25°C (P< 0.05). The 3% acetic acid incorporated
with 1.5% of cardamom showed the highest (P< 0.05) Total Phenolic Content (4.87±0.03 mg gallic
acid equivalent ImL) and Total Flavonoid Content (2.07±0.02 catechin equivalent I mL) and DPPH
radical scavenging activity (9.81 !.l mollg ±0.38) followed by 2% of Cinnamon added sample.
Sensory panelist were preferred 3% acetic acid incorporated 2% cinnamon> 1.5% cardamom. It
took 4 weeks for the production of spiced condiment.
Optimization of alcohol fermentation process in the 2nd study was carried out with three-sugar
concentration (16, 18 and 22°brix) and three methods of sugar addition (adding sugar at once,
