Abstract:
Phthalic Acid Esters (PAEs) are a group of emerging contaminants that are widely used as a plasticizer in
manufacturing polyethylene terephthalate (PET) bottles to improve softness, flexibility, durability,
longevity and workability. Some of PAEs have, however, been identified to be carcinogenic. People in Sri
Lanka often use the PET bottles for storing ambient and warm water repeatedly for subsequent
consumption. With the reuse of bottles, PAEs are easily detached and migrated to the water, because of
weak chemical bonding between the main polymer structure and PAEs. However, no comprehensive
studies on the migration of PAEs from the material of the bottle to water in the context of different reuse
efforts of storing ambient and warm water have been reported; hence, this study. Ten different
commercial brands of PET bottles were selected. The initial storage temperature of selected PET bottles
was measured and they were under room temperature conditions. It was ensured that they were not
exposed to the direct sunlight under storage condition. Three bottles of each brand were filled with warm
water (initial temperature 60±2º C) and another three with the same water at room temperature (27±2 ºC)
and kept for 12 hours. The PAE levels of the water in the bottles were analysed by Gas Chromatography-
Mass Spectrometer (GC-MS) using EPA method 8061A. Six PAEs, bis (2-ethylhexyl) phthalate (DEHP),
dibutyl phthalate (DBP), diethyl phthalate (DEP), benzylbutyl phthalate (BBP), dimethyl phthalate
(DMP) and di-n-octyl phthalate (DnOP) were quantified.The same bottles were subject to PAE analyses
for six consecutive reuse events. Characterisation of PET bottles of two brands was done to investigate
the thermally induced structural changes using the Fourier-Transform Infrared spectroscopy, Scanning
Electron Microscopy, Differential Scanning Calorimetry and X-Ray powder Diffraction.In general, DMP,
DEP, DnBP, BBP, and, DnOP were not detected in the water under room temperature (27±2º C) and
60±2º C. Among the six different PAEs tested, DEHP was the only PAE that has leached out at room
temperature and at 60ºC for different brands. Results manifested the maximum and minimum levels of
DEHP being migrated after each event of reuse at room temperature to be 82±4 μgL-1 and 17±2 μgL-1,
respectively, and the maximum and minimum levels of DEHP leached out at 60±2 ºC were 133±3 μgL-1
and 26±2 μgL-1, respectively. Characterisation studies indicate that there were structural changes with
each reuse event at 60±2 ºC in comparison to those at room temperature. In conclusion, this study
provides evidence of migration of DEHP from the material of the bottle to water in the reuse efforts for
storing warm water for subsequent consumption. Hence, regulating the quantities of DEHP to be added
during the manufacturing process of PET bottles and the reuse efforts of empty PET bottles is of utmost
importance to minimize the potential health and environmental impacts.
