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Microbial Degradation of Nitrate: Put Microbes to Work

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dc.contributor.author Gunasekara, N.D.
dc.contributor.author Idroos, F.S
dc.contributor.author Pathmalal, M.M.
dc.date.accessioned 2019-02-25T03:53:14Z
dc.date.available 2019-02-25T03:53:14Z
dc.date.issued 2019
dc.identifier.citation Gunasekara ND et al., Environment and Natural Resources Journal 2019; 17(2): 30-40 en_US
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/8347
dc.description.abstract Three nitrate degrading bacteria, namely S1, S2 and S3 strains, were isolated from soil samples collected from agricultural sites at Polonnaruwa, Oruwala and Gampaha, Sri Lanka respectively. Among the isolated strains, S1 showed a maximum nitrate removal rate of 4.20±0.08 mg/L/day whereas S2 and S3 showed nitrate removal rates of 3.45±0.57 mg/L/day and 3.72±0.19 mg/L/day, respectively. The nitrate removal abilities of all three strains were measured at different temperatures (25oC, 30oC, 32oC), different pH (7.0, 7.5, 8.0) and for different nitrate concentrations (15, 30, 45 mg/L) in vitro. The maximum nitrate degrading rate by the bacterium S1 (4.52±0.01 mg/L/day), S2 (4.25±0.47 mg/L/day) and S3 (4.04±0.09 mg/L/day) were detected at 32ºC whereas the highest degradation rate for S1 (4.6±0.05 mg/L/day), S2 (4.38±0.03 mg/L/day) and S3 (4.14±0.25 mg/L/day) bacteria strains was reported when the pH was 8.0. The maximum nitrate degradation was found to be 5.88±0.17, 5.67±0.45 and 5.71±0.43 mg/L/day for S1, S2 and S3 bacteria strains when 45 mg/L of nitrate was present in the medium. A biochemical test tentatively identified the isolates S1, S2 and S3 as Pseudomonas sp., Bacillus sp. and Proteus sp. The bio sand filter developed in co-operating all three bacterial strains showed 6.12±0.06 mg/L/day of nitrate degradation rate within 24 hours. en_US
dc.language.iso en en_US
dc.subject Nitrate, Nitrate degrading bacteria, Optimization, Bio-sand filter en_US
dc.title Microbial Degradation of Nitrate: Put Microbes to Work en_US
dc.type Article en_US
dc.identifier.doi https://doi.org/10.32526/ennrj.17.2.2019.10 en_US


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