Association of tumor necrosis factor alpha gene polymorphisms with Helicobacter pylori infection in dyspeptic patients in Sri Lanka

Abstract

Single nucleotide polymorphisms present on the promoter sequence of the TNF-a gene may affect production ofTNF-a, a pro-inflammatory cytokine, duringimmune responses. The presence of TNF-a polymorphisms is also reportedly associated with more severe manifestations of Helicobacter pylori infection. However, the frequency of TNF-a polymorphisms and the associated disease severity vary between different patient groups. In this study, gastric biopsies and blood specimens were collected from 138 patients with dyspepsia undergoing routine upper gastrointestinal endoscopy. Our institution's Ethics Review Committee approved the study and written informed consent was obtained from all participants. The presence of H. pylori was confirmed histologically in all patients. The frequency of TNF-a polymorphisms in the study cohort was investigated using PCR–restriction fragment length polymorphism and expression of serumTNF-aquantitated using a commercial ELISA assay.The proportions of selectedTNF-a polymorphisms (TNF-a-238, -308 and -863) were similar in H. pylori-positive and -negative patients. Homozygous mutations of TNF-a polymorphisms were rarely detected in the study group. There was a significant difference in TNF-a concentrations between patients with mild chronic gastritis and TNF-a -308 GG genotype and patients with moderate to severe chronic gastritis (P¼0.008). It was not possible to identify an association between these genotypes and disease severity because of the low frequency of heterozygous and homozygous mutated genes in Sri Lankan patients with dyspepsia.