Abstract:
An efficient plant regeneration system was
developed for Hordeum vulgare L. cv Baegdong - an important
high yielding Korean cultivar. This cultivar is resistant to
Fusarium and a few other fungi but susceptible to Barley Yellow
Mosaic Virus (BaYMV) and Barley Mild Mosaic Virus
(BaMMV). The protocol was based on a series of experiments
involving the sizes of immature embryos and the culture media.
The embryo size is found to be critical for the establishment of
callus. Embryos of 1.6-2.0 mm size showed the highest ability
to produce callus capable of regenerating green plants. The
auxins picloram and dicamba proved effective in inducing callus
from immature embryos. 2.5 mg L 1 dicamba and 4.0 mg L"1
picloram in Murashige and Skoog (MS) medium was optimum
for the induction of primary callus. The induced primary callus
was loose and friable which ultimately developed into creamy
white and compact callus after transferring into the fresh
medium. Multiple shoots were induced in the ViMS
medium supplemented with 6.0 g L"1 maltose, 20.0 g L"1 sorbitol,
0.5 mg L"1 2, 4-D and 1.0 mg L 1 kinetin and the rate was 6.5
shoots per embryo. Regenerated plants were hardy and
developed roots rapidly in the medium containing 0.2 mg L"1
Indolebutyric acid (IBA). This efficient plant regeneration
system provides a foundation for generating transgenic plants
of this important barley cultivar.
