EFFECT OF MIXED BACTERIAL CONSORTIA FOR DECOLORIZATION AND DETOXIFICATION OF AN AZO DYE: CI DIRECT BLUE 201 TEXTILE DYE

Abstract

Synthetic textile dyes generated from textile industry, has become a severe environmental problem due to the xenobiotic properties and toxicity of the dyes. The present study was focussed on the potential application of different bacterial consortium consists with Alcaligenes faecalis (MK166784), Micrococcus luteus (MK166783) and Staphylococcus warneri (MK256311) in A-1 (A. faecalis, M. luteus and S. warneri), A-2 (A. faecalis and M. luteus), A-3 (A. faecalis and S. warneri) and A-4 (M. luteus and S. warneri) consortia for the decolorization of CI Direct Blue 201, sulfonated azo dye. Among the different bacterial consortia tested, the consortium A-2 was found to be the best bacterial consortium for the decolorization of CI Direct Blue 201 dye, than the monocultures of bacteria employed itself. The consortium (A2) achieved complete decolorization of CI Direct Blue 201 textile dye at 48 hrs of incubation under static conditions. Futher, it was found that the bacterial consortium A-2 utilized the dyes efficiently with the supplement of tryptone and peptone as the co-substrates in modified Mineral Salt Medium. Results of the phytotoxicity assay confirmed that the dye was detoxified after the treatment. Thus the treated dye contained water can be used for agrarian purposes.