dc.contributor.author |
Weerasooriya, M.K.B. |
|
dc.contributor.author |
Simpson, T.J. |
|
dc.date.accessioned |
2013-04-05T05:08:31Z |
|
dc.date.available |
2013-04-05T05:08:31Z |
|
dc.date.issued |
2000 |
|
dc.identifier.citation |
Weerasooriya, M.K.B., & Simpson, T.J. (2000). Purification of NADP Dependent, 1,3,6,8 -Tetrahydroxynaphthalene Reductase from Verticillium dahliae brm - 1(ATCC 44571). Vidyodaya Journal of Science, 9, 1-13. |
en-US |
dc.identifier.uri |
http://dr.lib.sjp.ac.lk/handle/123456789/984 |
|
dc.description.abstract |
NADPH dependent 1,3,6,8- tetrahydroxynaphthalene Reductase, one
of the key enzymes of 1,8-dihydroxy melanin (DHN melanin) biosynthesis
was isolated from verticillium dahliae brm-I (ATCC 44571) and purified to
near homogeneity (as shown by SDS-PAGE), using ammonium sulphate
fractionation, ion exchange chromatography on DEAE Cellulose, FPLC
Mono Q anion exchange and Superose - 12 gel filtration chromatography,
Fold purification and yield of the enzyme were found to be 1423 and 0.47%
respectively, Specific activity ofthe final enzyme fraction was 79,7 (nmole/
mg.min) Approximate molecular weight of the purified enzyme was determined
as-24 kDa by Superose-12 gel filtration chromatography,
A satisfactory HPLC based enzyme assay was also developed for thepurification of enzyme |
en_US |
dc.language.iso |
en |
en_US |
dc.subject |
Melanin biosynthesis |
en_US |
dc.subject |
Verticillium dahliae brm-l |
en_US |
dc.subject |
Tetrahydroxynaphthalene reductase |
en_US |
dc.subject |
Purification |
en_US |
dc.title |
Purification of NADP Dependent, 1,3,6,8 -Tetrahydroxynaphthalene Reductase from Verticillium dahliae brm - 1(ATCC 44571) |
en_US |
dc.type |
Article |
en_US |
dc.date.published |
2000 |
|