Characterisation of a Geminivirus Infecting Cassava in Sri Lanka

dc.contributor.author	Salim, N.
dc.contributor.author	Bandumala, S.H.
dc.date.accessioned	2013-04-22T03:57:33Z
dc.date.available	2013-04-22T03:57:33Z
dc.date.issued	2001
dc.identifier.citation	Salim, N., & Bandumala, S.H. (2001). Characterisation of a Geminivirus Infecting Cassava in Sri Lanka. Vidyodaya Journal of Science, 10, 151-165.	en-US
dc.identifier.uri	http://dr.lib.sjp.ac.lk/handle/123456789/996
dc.description.abstract	A geminivirus, related to Indian cassava mosaic virus (ICMV) was detected in fifteen samples of cassava collected from different areas of the island. The virus was transmitted by sap inoculation to herbaceous hosts and graft inoculation to cassava. The isolates tested showed a narrow host range. The virus was detected in many varities of Manihot esculenta and ceara rubber (wild cassava) by enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). The virus was purified from systemically infected Nicotiana benthamiana leaves. Geminate particles of 18-20x 30 nm in size were observed by electron microscopy. An atiserum was prepared and the titre was found to be 1/32 as determined by gel double diffusion. This polyclonal antiserum detected geminiviruses of Acalypha mosaic disease and Ageratum yellow vein disease but not the geminivirus of Hibiscus yellow vein disease in indirect ELISA.	en_US
dc.language.iso	en	en_US
dc.subject	Acalypha	en_US
dc.subject	Ageratum	en_US
dc.subject	Geminiviruses	en_US
dc.subject	Hibiscus	en_US
dc.subject	Indian cassava mosaic virus	en_US
dc.title	Characterisation of a Geminivirus Infecting Cassava in Sri Lanka	en_US
dc.type	Article	en_US
dc.date.published	2001