Abstract:
Three cloned highly repetitive DNA sequences Rp36, Rp2l7 and Rp234
isolated from An. culicifacies Giles, sensu lato were developed as non
radioactive DNA probes by using a biotinylated labeling and colorimetric
detection system. These non radioactive DNA probes distinguish sibling
species A from species Band C of the An. culicifacies complex in a dot blot
hybridization assay using single mosquito DNA extracts diluted 50 fold. The
biotinylated Rp217 probe was further assayed in a more simple procedure
which involves the hybridization of blots prepared from squashed mosquito
heads. This technique avoids the separate extraction of mosquito DNA and
facilitates a number of samples to be processed rapidly while also allowing
several field analyses to be carried out on one mosquito specimen.
