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A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex

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dc.contributor.author De Silva, B.G.D.N.K.
dc.contributor.author Hapugoda, G.P.G.M.D.
dc.contributor.author Karunanayake, E.H.
dc.date.accessioned 2013-04-24T05:24:37Z
dc.date.available 2013-04-24T05:24:37Z
dc.date.issued 2010
dc.identifier.citation De Silva, B.G.D.N.K., Hapugoda, G.P.G.M.D., & Karunanayake, E.H. (2010). A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex. Vidyodaya Journal of Science, 15(1&2), 63-72. en-US
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/1014
dc.description.abstract Three cloned highly repetitive DNA sequences Rp36, Rp2l7 and Rp234 isolated from An. culicifacies Giles, sensu lato were developed as non radioactive DNA probes by using a biotinylated labeling and colorimetric detection system. These non radioactive DNA probes distinguish sibling species A from species Band C of the An. culicifacies complex in a dot blot hybridization assay using single mosquito DNA extracts diluted 50 fold. The biotinylated Rp217 probe was further assayed in a more simple procedure which involves the hybridization of blots prepared from squashed mosquito heads. This technique avoids the separate extraction of mosquito DNA and facilitates a number of samples to be processed rapidly while also allowing several field analyses to be carried out on one mosquito specimen. en_US
dc.language.iso en en_US
dc.subject Anopheles culicifacies en_US
dc.subject Non radioactive en_US
dc.subject DNA probes en_US
dc.title A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex en_US
dc.type Article en_US
dc.date.published 2010


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