dc.contributor.author |
De Silva, B.G.D.N.K. |
|
dc.contributor.author |
Hapugoda, G.P.G.M.D. |
|
dc.contributor.author |
Karunanayake, E.H. |
|
dc.date.accessioned |
2013-04-24T05:24:37Z |
|
dc.date.available |
2013-04-24T05:24:37Z |
|
dc.date.issued |
2010 |
|
dc.identifier.citation |
De Silva, B.G.D.N.K., Hapugoda, G.P.G.M.D., & Karunanayake, E.H. (2010). A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex. Vidyodaya Journal of Science, 15(1&2), 63-72. |
en-US |
dc.identifier.uri |
http://dr.lib.sjp.ac.lk/handle/123456789/1014 |
|
dc.description.abstract |
Three cloned highly repetitive DNA sequences Rp36, Rp2l7 and Rp234
isolated from An. culicifacies Giles, sensu lato were developed as non
radioactive DNA probes by using a biotinylated labeling and colorimetric
detection system. These non radioactive DNA probes distinguish sibling
species A from species Band C of the An. culicifacies complex in a dot blot
hybridization assay using single mosquito DNA extracts diluted 50 fold. The
biotinylated Rp217 probe was further assayed in a more simple procedure
which involves the hybridization of blots prepared from squashed mosquito
heads. This technique avoids the separate extraction of mosquito DNA and
facilitates a number of samples to be processed rapidly while also allowing
several field analyses to be carried out on one mosquito specimen. |
en_US |
dc.language.iso |
en |
en_US |
dc.subject |
Anopheles culicifacies |
en_US |
dc.subject |
Non radioactive |
en_US |
dc.subject |
DNA probes |
en_US |
dc.title |
A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex |
en_US |
dc.type |
Article |
en_US |
dc.date.published |
2010 |
|