Attached
Introduction and objectives
This study aimed to assess the association of
HLA alleles; HLA-DQA1*0102, HLADQA1*0103 and HLA-DQB1*0301, with the
presence of H .p y lo r i infection and disease
severity among dyspeptic patients.
Method
Gastric tissue samples from 100 dyspeptic
patients, who underwent upper gastrointestinal endoscopy at Colombo South
Teaching Hospital were included. Presence of
selected HLA alleles were confirmed using PCR
(Polymerase Chain Reaction). H .p y lo r i
infection was determined using PCR and
Histology. The histological interpretation was
done according to the "Sydney classification".
i
Results
Out of 100 patients, respective percentages of
HLA-DQA1*0102, HLA-DQA1*0103 and HLADQB1*0301 were 39, 32 and 20 respectively.
Of these, 25 were positive for both DQA1 alleles, and 4 were positive for all three.
Presence of H. pylori was confimed in 22
biopsies by PCR and in 13 biopsies by
Histological examination. Out of 22 H.pylori
PCR positive patients, presence of alleles
DQA1*0102, DQA1*0103 and 0061*0301
were 50.0%(ll/22), 36.4%(8/22) and
9.1%(2/22) respectively. By histology,
53.8%(7/13), 23.196(3/13) and 15.1%(2/13)
were identified to have HLA-DQA1*0102, HLADQA1*0103 and HLA-DQBl*0301respectively.
Most of the samples showed only mild chronic
inflammation. Out of the 20 samples positive
for HLA-DQBl*0301,two samples had
moderate inflammation and another sample
was positive for intestinal metaplasia.
Spearman rank correlation coefficient analysis
of HLA-DQA1*0102 showed a weak positive
correlation (p=0.118) and DQA1*0103 (p= -
.066) and DQB1*0301 (p= -.045)showed a
weak negative correlation, respectively for
H.pylori infection.
Conclusions
H. pylori infection had a weak correlation to
the selected HLA alleles in the local
population. No significant association
between disease severity and HLA alleles were
observed.
