Association of selected HLA DQA2 and OQB1 alleles with Helicobacter pylori infection and disease severity among dyspeptic patients

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Introduction and objectives This study aimed to assess the association of HLA alleles; HLA-DQA1*0102, HLADQA1*0103 and HLA-DQB1*0301, with the presence of H .p y lo r i infection and disease severity among dyspeptic patients. Method Gastric tissue samples from 100 dyspeptic patients, who underwent upper gastrointestinal endoscopy at Colombo South Teaching Hospital were included. Presence of selected HLA alleles were confirmed using PCR (Polymerase Chain Reaction). H .p y lo r i infection was determined using PCR and Histology. The histological interpretation was done according to the "Sydney classification". i Results Out of 100 patients, respective percentages of HLA-DQA1*0102, HLA-DQA1*0103 and HLADQB1*0301 were 39, 32 and 20 respectively. Of these, 25 were positive for both DQA1 alleles, and 4 were positive for all three. Presence of H. pylori was confimed in 22 biopsies by PCR and in 13 biopsies by Histological examination. Out of 22 H.pylori PCR positive patients, presence of alleles DQA1*0102, DQA1*0103 and 0061*0301 were 50.0%(ll/22), 36.4%(8/22) and 9.1%(2/22) respectively. By histology, 53.8%(7/13), 23.196(3/13) and 15.1%(2/13) were identified to have HLA-DQA1*0102, HLADQA1*0103 and HLA-DQBl*0301respectively. Most of the samples showed only mild chronic inflammation. Out of the 20 samples positive for HLA-DQBl*0301,two samples had moderate inflammation and another sample was positive for intestinal metaplasia. Spearman rank correlation coefficient analysis of HLA-DQA1*0102 showed a weak positive correlation (p=0.118) and DQA1*0103 (p= - .066) and DQB1*0301 (p= -.045)showed a weak negative correlation, respectively for H.pylori infection. Conclusions H. pylori infection had a weak correlation to the selected HLA alleles in the local population. No significant association between disease severity and HLA alleles were observed.